|Title||Internal ribosome entry sites and dicistronic RNAs in mammalian transgenesis.|
|Publication Type||Journal Article|
|Year of Publication||1995|
|Authors||Mountford P, Smith AG|
|Date Published||1995 May|
|Keywords||Animals, Animals, Genetically Modified, Humans, Mammals, Ribosomes, RNA|
Modification of the genetic content of cultured cells or of whole animals is now a key strategy in both basic biological research and applied biotechnology. Yet obtaining the desired level and specificity of expression of an introduced gene remains highly problematic. One solution could be to couple expression of a transgene to that of an appropriate intact genomic locus. The identification and functional characterization of RNA sequences known as internal ribosome entry sites now offer the possibility of achieving precise control of transgene expression through the generation of dicistronic fusion mRNAs.
|Alternate Journal||Trends Genet.|