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Influence of menstrual cycle on circulating endothelial progenitor cells.

TitleInfluence of menstrual cycle on circulating endothelial progenitor cells.
Publication TypeJournal Article
Year of Publication2009
AuthorsRobb AO, Mills NL, Smith IBJ, Short A, Tura-Ceide O, Barclay GR, Blomberg A, Critchley HOD, Newby DE, Denison FC
JournalHum Reprod
Volume24
Issue3
Pagination619-25
Date Published2009 Mar
ISSN1460-2350
KeywordsAdult, Antigens, CD, Antigens, CD34, Endothelial Cells, Endothelium, Vascular, Female, Flow Cytometry, Glycoproteins, Humans, Immunophenotyping, Menstrual Cycle, Neovascularization, Pathologic, Peptides, Stem Cells, Steroids, Vascular Endothelial Growth Factor Receptor-2
Abstract

BACKGROUND: Endothelial progenitor cells (EPCs) are circulating mononuclear cells that participate in angiogenesis. The aim of this study was to determine the influence of the menstrual cycle on the number and function of EPCs, and to investigate their relationship with circulating concentrations of sex steroids and inflammatory mediators.

METHODS: Ten healthy nulliparous, premenopausal, non-smoking women with regular menses were studied over a single menstrual cycle. Venepuncture was performed in the menstrual, follicular, peri-ovulatory and luteal phases. EPCs were quantified by flow cytometry (CD133(+)CD34(+)KDR(+) phenotype) and the colony-forming unit (CFU-EPC) functional assay. Circulating concentrations of estradiol, progesterone and inflammatory mediators (TNF-alpha, IL-6, sICAM-1 and VEGF) were measured by immunoassays.

RESULTS: The numbers of CD133(+)CD34(+)KDR(+) cells were higher in the follicular phase (0.99 +/- 0.3 x 10(6) cells/l) compared with the peri-ovulatory phase (0.29 +/- 0.1 x 10(6) cells/l; P

CONCLUSIONS: CD133(+)CD34(+)KDR(+) cells but not CFU-EPCs vary during the menstrual cycle. Our findings suggest a potential role for circulating EPCs in the normal cycle of physiological angiogenesis and repair of the uterine endometrium that is independent of circulating sex steroids or inflammatory mediators.

DOI10.1093/humrep/den411
Alternate JournalHum. Reprod.
PubMed ID19088108