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Infertility in a transgenic rat due to impairment of cytoplasmic elimination and sperm release from the Sertoli cells.

TitleInfertility in a transgenic rat due to impairment of cytoplasmic elimination and sperm release from the Sertoli cells.
Publication TypeJournal Article
Year of Publication1995
AuthorsSharpe RM, Maguire SM, Saunders PT, Millar MR, Russell LD, Ganten D, Bachmann S, Mullins L, Mullins JJ
JournalBiol Reprod
Volume53
Issue1
Pagination214-26
Date Published1995 Jul
ISSN0006-3363
KeywordsAnimals, Animals, Genetically Modified, Base Sequence, Blotting, Northern, Cytoplasm, Epididymis, Immunohistochemistry, In Situ Hybridization, Infertility, Male, Male, Microscopy, Electron, Molecular Sequence Data, Rats, Renin, RNA, Messenger, Sertoli Cells, Spermatozoa, Testis
Abstract

In line TGR(mRen2)26 transgenic rats (TGR26) bearing a randomly inserted additional renin transgene, the males, but not the females, were found to be infertile. Tissue was obtained from TGR26 males and littermate controls after perfusion fixation, and the morphology of the testes and epididymides was examined. Testis size was normal as was gross morphology, but careful examination revealed that the release of many spermatozoa at stage IX of the spermatogenic cycle was impaired. In addition, the process of cytoplasmic elimination was abnormal, as cytoplasmic fragments of elongate spermatids were present in the epididymis. In TGR26 males, seminiferous tubule lumen size was significantly larger (p

Alternate JournalBiol. Reprod.
PubMed ID7669852