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Human myogenic endothelial cells exhibit chondrogenic and osteogenic potentials at the clonal level.

TitleHuman myogenic endothelial cells exhibit chondrogenic and osteogenic potentials at the clonal level.
Publication TypeJournal Article
Year of Publication2013
AuthorsZheng B, Li G, Chen WCW, Deasy BM, Pollett JB, Sun B, Drowley L, Gharaibeh B, Usas A, Péault B, Huard J
JournalJ Orthop Res
Date Published2013 Jul
KeywordsAdipocytes, Adult, Animals, Biopsy, Cell Differentiation, Cell Proliferation, Cells, Cultured, Chondrocytes, Chondrogenesis, Endothelium, Humans, Male, Mice, Mice, SCID, Muscle Development, Muscle, Skeletal, Osteoblasts, Osteogenesis, Oxidative Stress, Transplantation, Heterologous

We have previously reported the high regenerative potential of murine muscle-derived stem cells (mMDSCs) that are capable of differentiating into multiple mesodermal cell lineages, including myogenic, endothelial, chondrocytic, and osteoblastic cells. Recently, we described a putative human counterpart of mMDSCs, the myogenic endothelial cells (MECs), in adult human skeletal muscle, which efficiently repair/regenerate the injured and dystrophic skeletal muscle as well as the ischemic heart in animal disease models. Nevertheless it remained unclear whether human MECs, at the clonal level, preserve mMDSC-like chondrogenic and osteogenic potentials and classic stem cell characteristics including high proliferation and resistance to stress. Herein, we demonstrated that MECs, sorted from fresh postnatal human skeletal muscle biopsies, can be grown clonally and exhibit robust resistance to oxidative stress with no tumorigeneity. MEC clones were capable of differentiating into chondrocytes and osteoblasts under inductive conditions in vitro and participated in cartilage and bone formation in vivo. Additionally, adipogenic and angiogenic potentials of clonal MECs (cMECs) were observed. Overall, our study showed that cMECs not only display typical properties of adult stem cells but also exhibit chondrogenic and osteogenic capacities in vitro and in vivo, suggesting their potential applications in articular cartilage and bone repair/regeneration.

Alternate JournalJ. Orthop. Res.
PubMed ID23553740
Grant ListG1000816 / / Medical Research Council / United Kingdom
IU54AR050733-01 / AR / NIAMS NIH HHS / United States
R01-AR049684 / AR / NIAMS NIH HHS / United States
R01-DE13420-06 / DE / NIDCR NIH HHS / United States
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