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Histochemical and ultrastructural modification of mucosal mast cell granules in parasitized mice lacking the beta-chymase, mouse mast cell protease-1.

TitleHistochemical and ultrastructural modification of mucosal mast cell granules in parasitized mice lacking the beta-chymase, mouse mast cell protease-1.
Publication TypeJournal Article
Year of Publication1998
AuthorsWastling JM, Knight P, Ure J, Wright S, Thornton EM, Scudamore CL, Mason J, Smith A, Miller HR
JournalAm J Pathol
Volume153
Issue2
Pagination491-504
Date Published1998 Aug
ISSN0002-9440
KeywordsAnimals, Cell Count, Chymases, Connective Tissue, Enzyme-Linked Immunosorbent Assay, Esterases, Immunity, Mucosal, Immunoenzyme Techniques, Inclusion Bodies, Intestinal Mucosa, Mast Cells, Mice, Mice, Inbred C57BL, Mice, Knockout, Microscopy, Electron, Nippostrongylus, Polymerase Chain Reaction, Serine Endopeptidases, Strongylida Infections
Abstract

The soluble beta-chymases mouse mast cell protease-1 (mMCP-1) and rat mast cell protease-II are predominantly expressed by intestinal mucosal mast cells (IMMCs) and may promote mucosal epithelial permeability when released during intestinal allergic hypersensitivity responses. To study the function of these chymases, we generated mice with a homozygous null mutation of the mMCP-1 gene and investigated their response to infection with the intestinal nematode Nippostrongylus brasiliensis. Whereas mMCP-2, -4, and -5 were transcribed normally, there was no transcription of the mMCP-1 gene in null (-/-) mice, nor was mature mMCP-1 protein detected in (-/-) jejunal mucosa. In contrast, levels of mMCP-1 in wild-type (+/+) jejunal mucosa increased 200- to 350-fold from 0.66 microg mMCP-1/g wet weight in uninfected mice to 129 and 229 microg/g wet weight on days 8 and 10 of infection, respectively. The kinetics of IMMC recruitment differed in -/- mice compared with +/+ controls on days 8 (P

Alternate JournalAm. J. Pathol.
PubMed ID9708809
PubMed Central IDPMC1852988
Grant List / / Wellcome Trust / United Kingdom