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Generation of tissue organoids by compaction reaggregation.

TitleGeneration of tissue organoids by compaction reaggregation.
Publication TypeJournal Article
Year of Publication2014
AuthorsSheridan JM, C Blackburn C
JournalMethods Mol Biol
Volume1092
Pagination143-51
Date Published2014
ISSN1940-6029
KeywordsAnimals, Cell Aggregation, Mice, Molecular Biology, Organoids, Thymus Gland
Abstract

Cellular reaggregation methods are commonly used to generate tissue organoids for use in biological studies. Using a modified method termed "compaction reaggregation," it is possible to establish reaggregates of reproducible size from defined input cell numbers with ease and without specialist equipment. Importantly, this method is suitable for the study of tissues that have proved refractory to reaggregation by other methods. With the option of juxtaposing cell populations, this method is useful for studies of tissue organization and structure.

DOI10.1007/978-1-60327-292-6_9
Alternate JournalMethods Mol. Biol.
PubMed ID24318818
Grant List / / Medical Research Council / United Kingdom
Publication institute
CRM