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Generation of osteoblasts and chondrocytes from embryonic stem cells.

TitleGeneration of osteoblasts and chondrocytes from embryonic stem cells.
Publication TypeJournal Article
Year of Publication2006
AuthorsKawaguchi J
JournalMethods Mol Biol
Volume330
Pagination135-48
Date Published2006
ISSN1064-3745
KeywordsAnimals, Antineoplastic Agents, Cell Culture Techniques, Cell Differentiation, Cells, Cultured, Chondrocytes, Embryo, Mammalian, Embryo, Nonmammalian, Osteoblasts, Reverse Transcriptase Polymerase Chain Reaction, Stem Cells, Tretinoin
Abstract

The efficient generation of mesenchymal cells such as adipocytes, osteoblasts, and chondrocytes from embryonic stem cells is achieved by following sequential steps: embryoid body formation, retinoic acid (RA) treatment, and exposure to specific reagents for differentiation. RA treatment of embryoid bodies is critical for subsequent mesengenesis. Adipogenesis, osteogenesis, and chondrogenesis occur by culturing outgrowths for 2-3 wk with insulin/triiodothyronine, bone morphogenetic protein/dexamethasone-beta/glycerophosphate/ascorbic acid, and transforming growth factor-beta3/parathyroid hormone/1% fetal bovine serum, respectively. Emergence of these mesenchymal cells using a common initial procedure suggests that embryoid body formation and subsequent RA treatment results in the generation of a common progenitor for osteoblasts and chondrocytes.

DOI10.1385/1-59745-036-7:135
Alternate JournalMethods Mol. Biol.
PubMed ID16846022
Grant ListG0100724 / / Medical Research Council / United Kingdom
G19/38 / / Medical Research Council / United Kingdom