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Generation of embryonic stem cells and transgenic mice expressing green fluorescence protein in midbrain dopaminergic neurons.

TitleGeneration of embryonic stem cells and transgenic mice expressing green fluorescence protein in midbrain dopaminergic neurons.
Publication TypeJournal Article
Year of Publication2004
AuthorsZhao S, Maxwell S, Jimenez-Beristain A, Vives J, Kuehner E, Zhao J, O'Brien C, de Felipe C, Semina E, Li M
JournalEur J Neurosci
Volume19
Issue5
Pagination1133-40
Date Published2004 Mar
ISSN0953-816X
KeywordsAnimals, Cells, Cultured, Dopamine, Embryo, Mammalian, Female, Gene Expression Regulation, Developmental, Green Fluorescent Proteins, Homeodomain Proteins, Luminescent Proteins, Male, Mesencephalon, Mice, Mice, Inbred C57BL, Mice, Transgenic, Neurons, Pregnancy, Stem Cells, Transcription Factors
Abstract

We have generated embryonic stem (ES) cells and transgenic mice with green fluorescent protein (GFP) inserted into the Pitx3 locus via homologous recombination. In the central nervous system, Pitx3-directed GFP was visualized in dopaminergic (DA) neurons in the substantia nigra and ventral tegmental area. Live primary DA neurons can be isolated by fluorescence-activated cell sorting from these transgenic mouse embryos. In culture, Pitx3-GFP is coexpressed in a proportion of ES-derived DA neurons. Furthermore, ES cell-derived Pitx3-GFP expressing DA neurons responded to neurotrophic factors and were sensitive to DA-specific neurotoxin N-4-methyl-1, 2, 3, 6-tetrahydropyridine. We anticipate that the Pitx3-GFP ES cells could be used as a powerful model system for functional identification of molecules governing mDA neuron differentiation and for preclinical research including pharmaceutical drug screening and transplantation. The Pitx3 knock-in mice, on the other hand, could be used for purifying primary neurons for molecular studies associated with the midbrain-specific DA phenotype at a level not previously feasible. These mice would also provide a useful tool to study DA fate determination from embryo- or adult-derived neural stem cells.

DOI10.1111/j.1460-9568.2004.03206.x
Alternate JournalEur. J. Neurosci.
PubMed ID15016072