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Gene trap integrations expressed in the developing heart: insertion site affects splicing of the PT1-ATG vector.

TitleGene trap integrations expressed in the developing heart: insertion site affects splicing of the PT1-ATG vector.
Publication TypeJournal Article
Year of Publication1998
AuthorsMcClive P, Pall G, Newton K, Lee M, Mullins JJ, Forrester LM
JournalDev Dyn
Volume212
Issue2
Pagination267-76
Date Published1998 Jun
ISSN1058-8388
KeywordsAnimals, Artificial Gene Fusion, Base Sequence, Chromosome Mapping, DNA, Complementary, Embryonic and Fetal Development, Exons, Female, Fetal Heart, Genes, Reporter, Genetic Techniques, Genetic Vectors, Heart Defects, Congenital, Humans, Lac Operon, Male, Mice, Mice, Inbred C57BL, Mice, Transgenic, Molecular Sequence Data, Mutagenesis, Insertional, Phenotype, Pregnancy, RNA Splicing, Sequence Homology, Nucleic Acid, Stem Cells
Abstract

We describe the characterisation of three gene trap integrations in embryonic stem cells in which the lacZ reporter gene is repressed by retinoic acid (RA) in vitro and is expressed in the developing heart in vivo. In one of these, the gene trap vector has integrated into a gene that is located on chromosome 17 and is homologous to the human transcription factor gene, TFEB. Embryonic and adult cardiac expression of both the fusion transcript and the endogenous gene was confirmed. However, we show that the integration has not resulted in a null allele, because wild type transcripts, possibly resulting from splicing around the vector, are observed in homozygous tissue. The other two cardiac-expressing gene trap integrations have occurred into exons on chromosomes 1 and 5 and have used cryptic donor sites within the vector to generate functional fusion transcripts. One of these exon integrations results in a lethal neonatal phenotype.

DOI10.1002/(SICI)1097-0177(199806)212:2<267::AID-AJA11>3.0.CO;2-1
Alternate JournalDev. Dyn.
PubMed ID9626501