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Fibroblast growth factor induces a neural stem cell phenotype in foetal forebrain progenitors and during embryonic stem cell differentiation.

TitleFibroblast growth factor induces a neural stem cell phenotype in foetal forebrain progenitors and during embryonic stem cell differentiation.
Publication TypeJournal Article
Year of Publication2008
AuthorsPollard SM, Wallbank R, Tomlinson SR, Grotewold L, Smith A
JournalMol Cell Neurosci
Volume38
Issue3
Pagination393-403
Date Published2008 Jul
ISSN1095-9327
KeywordsAnimals, Cell Differentiation, Cell Line, Cells, Cultured, Embryonic Stem Cells, Female, Fibroblast Growth Factors, Genetic Markers, Mice, Mice, Inbred C57BL, Mice, Inbred CBA, Neurons, Phenotype, Pregnancy, Prosencephalon
Abstract

Neural stem (NS) cell lines may be derived via differentiation of pluripotent embryonic stem (ES) cells or from foetal forebrain. However, because NS cells arise in vitro from heterogeneous populations their immediate cellular origin remains unclear. We used microarray-based expression profiling to identify a set of markers expressed by mouse NS cells but not ES cells. One differentially expressed gene encodes the cell surface protein, CD44. CD44 expression is activated by FGF-2 in a subset of cells in both differentiating ES cells and foetal forebrain cultures. Following isolation by flow cytometry the CD44+ population was found to be highly enriched for NS cell founders. We found that other NS cell marker genes are also induced by FGF in culture, including: Adam12, Cadherin20, Cx3cl1, EGFR, Frizzled9, Kitl, Olig1, Olig2 and Vav3. We speculate that the self-renewing NS cell state may be generated in vitro following transcriptional resetting induced by FGF.

DOI10.1016/j.mcn.2008.03.012
Alternate JournalMol. Cell. Neurosci.
PubMed ID18504136
Grant ListG0800784 / / Medical Research Council / United Kingdom
G9806702 / / Medical Research Council / United Kingdom
/ / Biotechnology and Biological Sciences Research Council / United Kingdom