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Extensive hematopoietic stem cell generation in the AGM region via maturation of VE-cadherin+CD45+ pre-definitive HSCs.

TitleExtensive hematopoietic stem cell generation in the AGM region via maturation of VE-cadherin+CD45+ pre-definitive HSCs.
Publication TypeJournal Article
Year of Publication2008
AuthorsTaoudi S, le Gonneau C, Moore K, Sheridan JM, C Blackburn C, Taylor E, Medvinsky AL
JournalCell Stem Cell
Volume3
Issue1
Pagination99-108
Date Published2008 Jul 3
ISSN1875-9777
KeywordsAnimals, Antigens, CD, Antigens, CD45, Cadherins, Cell Culture Techniques, Cell Line, Colony-Forming Units Assay, Flow Cytometry, Genetic Markers, Hematopoietic Stem Cells, Humans, Reverse Transcriptase Polymerase Chain Reaction
Abstract

Elucidating the mechanisms underlying hematopoietic stem cell (HSC) specification and expansion in the embryo has been hampered by the lack of analytical cell culture systems that recapitulate in vivo development. Here, we describe an ex vivo model that facilitates a rapid and robust emergence of multipotent long-term repopulating HSCs in the embryonic AGM region. Because this method includes a cell dissociation step prior to reconstruction of a three-dimensional functional tissue and preserves both stromal and hematopoietic elements, it allowed us to identify the direct ancestry of the rapidly expanding HSC pool. We demonstrate that extensive generation of definitive HSCs in the AGM occurs predominantly through the acquisition of stem characteristics by the VE-cadherin+CD45+ population.

DOI10.1016/j.stem.2008.06.004
Alternate JournalCell Stem Cell
PubMed ID18593562
Grant ListG0500950 / / Medical Research Council / United Kingdom