Leading science, pioneering therapies
CRM Publications

Esrrb Complementation Rescues Development of Nanog-Null Germ Cells.

TitleEsrrb Complementation Rescues Development of Nanog-Null Germ Cells.
Publication TypeJournal Article
Year of Publication2018
AuthorsZhang M, Leitch HG, Tang WWC, Festuccia N, Hall-Ponsele E, Nichols J, M Surani A, Smith A, Chambers I
JournalCell Rep
Date Published2018 Jan 09

The transcription factors (TFs) Nanog and Esrrb play important roles in embryonic stem cells (ESCs) and during primordial germ-cell (PGC) development. Esrrb is a positively regulated direct target of NANOG in ESCs that can substitute qualitatively for Nanog function in ESCs. Whether this functional substitution extends to the germline is unknown. Here, we show that germline deletion of Nanog reduces PGC numbers 5-fold at midgestation. Despite this quantitative depletion, Nanog-null PGCs can complete germline development in contrast to previous findings. PGC-like cell (PGCLC) differentiation of Nanog-null ESCs is also impaired, with Nanog-null PGCLCs showing decreased proliferation and increased apoptosis. However, induced expression of Esrrb restores PGCLC numbers as efficiently as Nanog. These effects are recapitulated in vivo: knockin of Esrrb to Nanog restores PGC numbers to wild-type levels and results in fertile adult mice. These findings demonstrate that Esrrb can replace Nanog function in germ cells.

Alternate JournalCell Rep
PubMed ID29320730
Publication institute