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EphB-ephrin-B2 interactions are required for thymus migration during organogenesis.

TitleEphB-ephrin-B2 interactions are required for thymus migration during organogenesis.
Publication TypeJournal Article
Year of Publication2010
AuthorsFoster KE, Gordon J, Cardenas K, Veiga-Fernandes H, Makinen T, Grigorieva EF, Wilkinson DG, C Blackburn C, Richie E, Manley NR, Adams R, Kioussis D, Coles MC
JournalProc Natl Acad Sci U S A
Volume107
Issue30
Pagination13414-9
Date Published2010 Jul 27
ISSN1091-6490
KeywordsAnimals, Cell Movement, Embryo, Mammalian, Ephrin-B2, Female, Flow Cytometry, Immunohistochemistry, Male, Mesoderm, Mice, Mice, Knockout, Microscopy, Confocal, Nervous System, Organogenesis, Protein Binding, Receptors, Eph Family, Thymus Gland
Abstract

Thymus organogenesis requires coordinated interactions of multiple cell types, including neural crest (NC) cells, to orchestrate the formation, separation, and subsequent migration of the developing thymus from the third pharyngeal pouch to the thoracic cavity. The molecular mechanisms driving these processes are unclear; however, NC-derived mesenchyme has been shown to play an important role. Here, we show that, in the absence of ephrin-B2 expression on thymic NC-derived mesenchyme, the thymus remains in the cervical area instead of migrating into the thoracic cavity. Analysis of individual NC-derived thymic mesenchymal cells shows that, in the absence of ephrin-B2, their motility is impaired as a result of defective EphB receptor signaling. This implies a NC-derived cell-specific role of EphB-ephrin-B2 interactions in the collective migration of the thymic rudiment during organogenesis.

DOI10.1073/pnas.1003747107
Alternate JournalProc. Natl. Acad. Sci. U.S.A.
PubMed ID20616004
PubMed Central IDPMC2922182
Grant ListG0601156 / / Medical Research Council / United Kingdom
MC_U117532048 / / Medical Research Council / United Kingdom