|Title||DNA electrophoresis: historical and theoretical perspectives.|
|Publication Type||Journal Article|
|Year of Publication||2013|
|Authors||Roberts GA, Dryden DTF|
|Journal||Methods Mol Biol|
|Keywords||DNA, Electrophoresis, Agar Gel, Electrophoresis, Gel, Pulsed-Field, Humans, Sepharose|
The technique of gel electrophoresis is now firmly established as a routine laboratory method for analyzing DNA. Here, we describe the development of the methodology as well as a brief explanation of how the technique works. There is a short introduction to pulsed-field agarose gel electrophoresis, which represents a critical advancement in the method that facilitates the analysis of very large fragments of DNA. Finally, theoretical considerations are included.
|Alternate Journal||Methods Mol. Biol.|