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Distinct Wnt-driven primitive streak-like populations reflect in vivo lineage precursors.

TitleDistinct Wnt-driven primitive streak-like populations reflect in vivo lineage precursors.
Publication TypeJournal Article
Year of Publication2014
AuthorsTsakiridis A, Huang Y, Blin G, Skylaki S, Wymeersch FJ, Osorno R, Economou C, Karagianni E, Zhao S, Lowell S, Wilson V
JournalDevelopment
Volume141
Issue6
Pagination1209-21
Date Published2014 Mar
ISSN1477-9129
KeywordsAnimals, Cell Differentiation, Cell Lineage, Cells, Cultured, Gastrula, Gastrulation, Germ Layers, Mice, Mice, Transgenic, Neural Plate, Pluripotent Stem Cells, Primitive Streak, Wnt Signaling Pathway
Abstract

During gastrulation, epiblast cells are pluripotent and their fate is thought to be constrained principally by their position. Cell fate is progressively restricted by localised signalling cues from areas including the primitive streak. However, it is unknown whether this restriction accompanies, at the individual cell level, a reduction in potency. Investigation of these early transition events in vitro is possible via the use of epiblast stem cells (EpiSCs), self-renewing pluripotent cell lines equivalent to the postimplantation epiblast. Strikingly, mouse EpiSCs express gastrulation stage regional markers in self-renewing conditions. Here, we examined the differentiation potential of cells expressing such lineage markers. We show that undifferentiated EpiSC cultures contain a major subfraction of cells with reversible early primitive streak characteristics, which is mutually exclusive to a neural-like fraction. Using in vitro differentiation assays and embryo grafting we demonstrate that primitive streak-like EpiSCs are biased towards mesoderm and endoderm fates while retaining pluripotency. The acquisition of primitive streak characteristics by self-renewing EpiSCs is mediated by endogenous Wnt signalling. Elevation of Wnt activity promotes restriction towards primitive streak-associated lineages with mesendodermal and neuromesodermal characteristics. Collectively, our data suggest that EpiSC pluripotency encompasses a range of reversible lineage-biased states reflecting the birth of pioneer lineage precursors from a pool of uncommitted EpiSCs similar to the earliest cell fate restriction events taking place in the gastrula stage epiblast.

DOI10.1242/dev.101014
Alternate JournalDevelopment
PubMed ID24595287
PubMed Central IDPMC3943179
Grant ListG080297 / / Medical Research Council / United Kingdom
MR/K011200 / / Medical Research Council / United Kingdom