|Title||Differential stability of a human mini-chromosome in mouse cell lines.|
|Publication Type||Journal Article|
|Year of Publication||1998|
|Authors||Loupart ML, Shen MH, Smith A|
|Date Published||1998 Sep|
|Keywords||Animals, Cell Fusion, Centromere, CHO Cells, Chromosomes, Human, Cricetinae, Cricetulus, Electrophoresis, Gel, Pulsed-Field, Genetic Vectors, Humans, L Cells (Cell Line), Mice, Selection, Genetic, Species Specificity|
A 4 Mb human mini-chromosome, DeltaDelta2, was transferred from Chinese hamster ovary (CHO) cells into a mouse L cell line. The mini-chromosome could be transferred intact into the L cells, with 112/119 clones maintaining a mini-chromosome of the same size as the original. Ten clones were grown for 30 days in continuous culture. The mini-chromosomes were maintained stably with or without selection at a copy number of 1-2 per cell and none experienced any size alterations, as determined by pulsed-field gel electrophoresis. Thus DeltaDelta2 is structurally and mitotically stable in L cells. This contrasts with results in embryonic stem cells, in which DeltaDelta2 is highly unstable. These findings indicate that established somatic cell lines, such as L cells and CHO cells, have less stringent controls over centromeric function than do normal embryonic cells.
|Grant List||/ / Wellcome Trust / United Kingdom|