Title | Derivation of germline competent embryonic stem cells with a combination of interleukin-6 and soluble interleukin-6 receptor. |
Publication Type | Journal Article |
Year of Publication | 1994 |
Authors | Nichols J, Chambers I, Smith A |
Journal | Exp Cell Res |
Volume | 215 |
Issue | 1 |
Pagination | 237-9 |
Date Published | 1994 Nov |
ISSN | 0014-4827 |
Keywords | Animals, Blastocyst, Chimera, Ciliary Neurotrophic Factor, Culture Media, Serum-Free, Culture Techniques, Embryo Transfer, Female, Growth Inhibitors, Humans, Interleukin-6, Leukemia Inhibitory Factor, Lymphokines, Mice, Mice, Inbred C57BL, Mice, Inbred Strains, Nerve Tissue Proteins, Oncostatin M, Ovariectomy, Peptides, Rats, Receptors, Interleukin, Receptors, Interleukin-6, Recombinant Proteins, Stem Cells |
Abstract | In this report we document the derivation of pluripotential embryonic stem (ES) cells in the absence of a feeder layer by supplementation of culture media with either ciliary neurotrophic factor or oncostatin M, or with a combination of interleukin-6 (IL-6) plus soluble interleukin-6 receptor (sIL-6R). These factors all activate gp130-associated signaling processes, as does the previously characterized ES cell maintenance factor Differentiation Inhibiting Activity (Leukemia Inhibitory Factor). In particular, the IL-6/sIL-6R complex is thought to act exclusively through gp130. All ES cell lines derived using IL-6/sIL-6R contributed extensively to chimeras and were transmitted through the germline at high frequency. These findings point to a pivotal role for gp130 in ES cell propagation and may be relevant to attempts to derive ES cells from species other than mouse. |
DOI | 10.1006/excr.1994.1338 |
Alternate Journal | Exp. Cell Res. |
PubMed ID | 7957676 |