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Derivation of germline competent embryonic stem cells with a combination of interleukin-6 and soluble interleukin-6 receptor.

TitleDerivation of germline competent embryonic stem cells with a combination of interleukin-6 and soluble interleukin-6 receptor.
Publication TypeJournal Article
Year of Publication1994
AuthorsNichols J, Chambers I, Smith A
JournalExp Cell Res
Volume215
Issue1
Pagination237-9
Date Published1994 Nov
ISSN0014-4827
KeywordsAnimals, Blastocyst, Chimera, Ciliary Neurotrophic Factor, Culture Media, Serum-Free, Culture Techniques, Embryo Transfer, Female, Growth Inhibitors, Humans, Interleukin-6, Leukemia Inhibitory Factor, Lymphokines, Mice, Mice, Inbred C57BL, Mice, Inbred Strains, Nerve Tissue Proteins, Oncostatin M, Ovariectomy, Peptides, Rats, Receptors, Interleukin, Receptors, Interleukin-6, Recombinant Proteins, Stem Cells
Abstract

In this report we document the derivation of pluripotential embryonic stem (ES) cells in the absence of a feeder layer by supplementation of culture media with either ciliary neurotrophic factor or oncostatin M, or with a combination of interleukin-6 (IL-6) plus soluble interleukin-6 receptor (sIL-6R). These factors all activate gp130-associated signaling processes, as does the previously characterized ES cell maintenance factor Differentiation Inhibiting Activity (Leukemia Inhibitory Factor). In particular, the IL-6/sIL-6R complex is thought to act exclusively through gp130. All ES cell lines derived using IL-6/sIL-6R contributed extensively to chimeras and were transmitted through the germline at high frequency. These findings point to a pivotal role for gp130 in ES cell propagation and may be relevant to attempts to derive ES cells from species other than mouse.

DOI10.1006/excr.1994.1338
Alternate JournalExp. Cell Res.
PubMed ID7957676