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Derivation and propagation of embryonic stem cells in serum- and feeder-free culture.

TitleDerivation and propagation of embryonic stem cells in serum- and feeder-free culture.
Publication TypeJournal Article
Year of Publication2006
AuthorsNichols J, Ying Q-L
JournalMethods Mol Biol
Volume329
Pagination91-8
Date Published2006
ISSN1064-3745
KeywordsAnimals, Blastocyst, Cell Culture Techniques, Cell Separation, Chimera, Coculture Techniques, Culture Media, Serum-Free, Embryo, Mammalian, Female, Mice, Mice, Inbred C57BL, Pluripotent Stem Cells, Pregnancy
Abstract

The availability of murine embryonic stem (ES) cells has revolutionized the study of mammalian development and disease. We recently developed a culture medium that has enabled us to identify the essential signaling pathways required for maintenance of pluripotency in vitro. Addition of leukemia inhibitory factor and bone morphogenetic protein4 to this medium is sufficient to activate the signal transducer and activator of transcription3 and mammalian homolog of Drosophila mothers against decapentaplegic pathways, respectively. We have successfully derived and propagated ES cells in the absence of feeder cells and serum. This chapter describes a simple protocol for efficient derivation and maintenance of ES cells from embryos of the 129 and C57B1/6 strains of mice.

DOI10.1385/1-59745-037-5:91
Alternate JournalMethods Mol. Biol.
PubMed ID16845986
Grant ListG0300058 / / Medical Research Council / United Kingdom