Leading science, pioneering therapies
CRM Publications

Cumulus gene expression as a predictor of human oocyte fertilisation, embryo development and competence to establish a pregnancy.

TitleCumulus gene expression as a predictor of human oocyte fertilisation, embryo development and competence to establish a pregnancy.
Publication TypeJournal Article
Year of Publication2009
AuthorsAnderson RA, Sciorio R, Kinnell H, Bayne R, Thong KJ, de Sousa P, Pickering S
JournalReproduction
Volume138
Issue4
Pagination629-37
Date Published2009 Oct
ISSN1741-7899
KeywordsCumulus Cells, Embryonic Development, Female, Fertilization in Vitro, Gene Expression, Humans, Infertility, Female, Male, Oocytes, Oogenesis, Pregnancy, Pregnancy Maintenance, Prognosis, Quality Control, Sensitivity and Specificity, Sperm Injections, Intracytoplasmic, Treatment Outcome
Abstract

The close relationship between cumulus cell function and oocyte developmental competence indicates that analysis of cumulus gene expression is a potential non-invasive method to aid embryo selection and IVF outcome. Cumulus was isolated from 674 oocytes from 75 women undergoing ICSI and gene expression analysed by quantitative RT-PCR. Cumulus expression of cyclooxygenase 2 (PTGS2) was higher with mature oocytes, whereas brain-derived neurotrophic factor (BDNF) was lower when fertilisation was normal. Expression levels of gremlin (GREM1) and BDNF were weak positive and negative predictors of embryo quality respectively. Ranking of GREM1 expression within cohorts of oocytes showed that oocytes associated with the highest GREM1 expression were more likely to be transferred or cryopreserved than discarded (49 vs 33%, P

DOI10.1530/REP-09-0144
Alternate JournalReproduction
PubMed ID19602522
Grant ListCZB/4/537 / / Chief Scientist Office / United Kingdom
MC_U127684439 / / Medical Research Council / United Kingdom
WBS U.1276.00.002.00001.01 / / Medical Research Council / United Kingdom