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Complementary tissue-specific expression of LIF and LIF-receptor mRNAs in early mouse embryogenesis.

TitleComplementary tissue-specific expression of LIF and LIF-receptor mRNAs in early mouse embryogenesis.
Publication TypeJournal Article
Year of Publication1996
AuthorsNichols J, Davidson D, Taga T, Yoshida K, Chambers I, Smith A
JournalMech Dev
Date Published1996 Jul
KeywordsAnimals, Embryonic and Fetal Development, Female, Growth Inhibitors, Interleukin-6, Leukemia Inhibitory Factor, Leukemia Inhibitory Factor Receptor alpha Subunit, Lymphokines, Male, Mice, Morpholines, Organ Specificity, Pregnancy, Receptors, Cytokine, Receptors, OSM-LIF, RNA, Messenger, Stem Cells

The maintenance of pluripotential embryonic stem (ES) cells is dependent on the cytokine LIF. This report documents the mRNA expression profiles of LIF and the two components of the LIF-receptor complex, LIF-R and gp130, during early mouse embryogenesis. These mRNAs were undetectable in 1- or 2-cell embryos, but all were present by the blastocyst stage. LIF transcripts were localised in the differentiated trophectoderm, and were absent from the pluripotential inner cell mass. In contrast, LIF-R mRNA was found in the inner cell mass but not in the trophectoderm. This complementary pattern of expression is suggestive of a paracrine coupling between stem cells and differentiated progeny at the earliest stage of mammalian development. After implantation, transcripts for all components were down-regulated in the embryo. High levels of LIF-R and gp130 mRNAs were observed in the deciduum, however. These dynamic, tissue-specific expression patterns are consistent with regulatory roles for LIF or related cytokines, both in the maintenance of pluripotency in the mouse embryo, and in development of the foeto-maternal interface.

Alternate JournalMech. Dev.
PubMed ID8843390