Leading science, pioneering therapies
CRM Publications

Characterizing embryonic gene expression patterns in the mouse using nonredundant sequence-based selection.

TitleCharacterizing embryonic gene expression patterns in the mouse using nonredundant sequence-based selection.
Publication TypeJournal Article
Year of Publication2003
AuthorsSousa-Nunes R, Rana AAhmed, Kettleborough R, Brickman JM, Clements M, Forrest A, Grimmond S, Avner P, Smith JC, Dunwoodie SL, Beddington RS
JournalGenome Res
Volume13
Issue12
Pagination2609-20
Date Published2003 Dec
ISSN1088-9051
KeywordsAnimals, Crosses, Genetic, DNA, Complementary, Endoderm, Gene Expression Profiling, Gene Expression Regulation, Developmental, Gene Library, Mice, Mice, Inbred C57BL, Mice, Inbred CBA, Sequence Analysis, DNA
Abstract

This article investigates the expression patterns of 160 genes that are expressed during early mouse development. The cDNAs were isolated from 7.5 d postcoitum (dpc) endoderm, a region that comprises visceral endoderm (VE), definitive endoderm, and the node-tissues that are required for the initial steps of axial specification and tissue patterning in the mouse. To avoid examining the same gene more than once, and to exclude potentially ubiquitously expressed housekeeping genes, cDNA sequence was derived from 1978 clones of the Endoderm library. These yielded 1440 distinct cDNAs, of which 123 proved to be novel in the mouse. In situ hybridization analysis was carried out on 160 of the cDNAs, and of these, 29 (18%) proved to have restricted expression patterns.

DOI10.1101/gr.1362303
Alternate JournalGenome Res.
PubMed ID14613977
PubMed Central IDPMC403803