Title | Characterizing embryonic gene expression patterns in the mouse using nonredundant sequence-based selection. |
Publication Type | Journal Article |
Year of Publication | 2003 |
Authors | Sousa-Nunes R, Rana AAhmed, Kettleborough R, Brickman JM, Clements M, Forrest A, Grimmond S, Avner P, Smith JC, Dunwoodie SL, Beddington RS |
Journal | Genome Res |
Volume | 13 |
Issue | 12 |
Pagination | 2609-20 |
Date Published | 2003 Dec |
ISSN | 1088-9051 |
Keywords | Animals, Crosses, Genetic, DNA, Complementary, Endoderm, Gene Expression Profiling, Gene Expression Regulation, Developmental, Gene Library, Mice, Mice, Inbred C57BL, Mice, Inbred CBA, Sequence Analysis, DNA |
Abstract | This article investigates the expression patterns of 160 genes that are expressed during early mouse development. The cDNAs were isolated from 7.5 d postcoitum (dpc) endoderm, a region that comprises visceral endoderm (VE), definitive endoderm, and the node-tissues that are required for the initial steps of axial specification and tissue patterning in the mouse. To avoid examining the same gene more than once, and to exclude potentially ubiquitously expressed housekeeping genes, cDNA sequence was derived from 1978 clones of the Endoderm library. These yielded 1440 distinct cDNAs, of which 123 proved to be novel in the mouse. In situ hybridization analysis was carried out on 160 of the cDNAs, and of these, 29 (18%) proved to have restricted expression patterns. |
DOI | 10.1101/gr.1362303 |
Alternate Journal | Genome Res. |
PubMed ID | 14613977 |
PubMed Central ID | PMC403803 |