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Characterization of the opposite-strand genes from the mouse bidirectionally transcribed HTF9 locus.

TitleCharacterization of the opposite-strand genes from the mouse bidirectionally transcribed HTF9 locus.
Publication TypeJournal Article
Year of Publication1991
AuthorsBressan A, Somma MP, Lewis J, Santolamazza C, Copeland NG, Gilbert DJ, Jenkins NA, Lavia P
JournalGene
Volume103
Issue2
Pagination201-9
Date Published1991 Jul 22
ISSN0378-1119
KeywordsAmino Acid Sequence, Animals, Base Sequence, Blotting, Southern, Crosses, Genetic, Exons, Gene Expression, Genes, Overlapping, Genetic Variation, Humans, Introns, Mice, Mice, Inbred C57BL, Molecular Sequence Data, Nuclear Proteins, Promoter Regions, Genetic, Proteins, ran GTP-Binding Protein, Repetitive Sequences, Nucleic Acid
Abstract

The mouse HTF9 locus contains two genes that are bidirectionally transcribed with opposite polarity from a shared CpG-rich island. Both genes were previously shown to be expressed in a housekeeping fashion in mouse. We have now determined the molecular organization of the genes over 12 kb surrounding the island. In addition, we show that the HTF9 locus resides in the proximal region of mouse chromosome 16. We have sequenced the cDNAs corresponding to both divergent transcripts. Both genes appear to code for novel proteins that are structurally unrelated to each other. Finally, we show that both genes are highly conserved and efficiently expressed in human cells.

Alternate JournalGene
PubMed ID1889746
Grant ListN01-CO-74101 / CO / NCI NIH HHS / United States