|Title||[Characteristics of the proliferative activity of the aggregates forming during recombination of the epithelium and mesenchyma of embryonic lungs from intact and carcinogen-treated mice].|
|Publication Type||Journal Article|
|Year of Publication||1984|
|Authors||Kolesnichenko TS, Antoshina EE, Medvinskiĭ AL|
|Date Published||1984 Sep-Oct|
|Keywords||Animals, Cell Aggregation, Cell Division, Epithelium, Lung, Lung Neoplasms, Mesoderm, Mice, Mice, Inbred A, Mice, Inbred C57BL, Urethane|
Methods of separation of the mouse embryonic lung mesenchyme (M) and epithelium (E) and obtaining homolinear and heterolinear organotypic aggregates of E and M from the intact and urethane-treated (transplacentally) A and C57BL mouse embryos. In the aggregates formed by E and M from the experimental A embryos, the index of labelled nuclei (3H-thymidine incorporation) in both the tissue components was several times that in the aggregates of E and M from the intact embryos. M from the experimental embryos aggregated with E from the intact embryos increased the proliferative activity of the latter 4 to 11 times, whereas M of the intact embryos aggregated with E of the experimental embryos decreased its proliferative activity to the normal level. A suggestion is put forward that the embryonic lung mesenchyme plays an important role in the realization of transplacental carcinogenic influences and in the development of lung epithelial tumours.