Leading science, pioneering therapies
CRM Publications

Cell cycle population effects in perturbation studies.

TitleCell cycle population effects in perturbation studies.
Publication TypeJournal Article
Year of Publication2014
AuthorsO'Duibhir E, Lijnzaad P, Benschop JJ, Lenstra TL, van Leenen D, Koerkamp MJAGroot, Margaritis T, Brok MO, Kemmeren P, Holstege FCP
JournalMol Syst Biol
Date Published2014

Growth condition perturbation or gene function disruption are commonly used strategies to study cellular systems. Although it is widely appreciated that such experiments may involve indirect effects, these frequently remain uncharacterized. Here, analysis of functionally unrelated Saccharyomyces cerevisiae deletion strains reveals a common gene expression signature. One property shared by these strains is slower growth, with increased presence of the signature in more slowly growing strains. The slow growth signature is highly similar to the environmental stress response (ESR), an expression response common to diverse environmental perturbations. Both environmental and genetic perturbations result in growth rate changes. These are accompanied by a change in the distribution of cells over different cell cycle phases. Rather than representing a direct expression response in single cells, both the slow growth signature and ESR mainly reflect a redistribution of cells over different cell cycle phases, primarily characterized by an increase in the G1 population. The findings have implications for any study of perturbation that is accompanied by growth rate changes. Strategies to counter these effects are presented and discussed.

Alternate JournalMol. Syst. Biol.
PubMed ID24952590
PubMed Central IDPMC4265054
Publication institute