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A cell autonomous function of Brachyury in T/T embryonic stem cell chimaeras.

TitleA cell autonomous function of Brachyury in T/T embryonic stem cell chimaeras.
Publication TypeJournal Article
Year of Publication1991
AuthorsRashbass P, Cooke LA, Herrmann BG, Beddington RS
JournalNature
Volume353
Issue6342
Pagination348-51
Date Published1991 Sep 26
ISSN0028-0836
KeywordsAnimals, Chimera, Chromosome Deletion, Embryo, Mammalian, Embryonic and Fetal Development, Glucose-6-Phosphate Isomerase, Mesoderm, Mice, Mice, Inbred C57BL, Mutation, Phenotype, Stem Cells
Abstract

Developmental genetics has shown that the Brachyury (T) gene has a key role in mesoderm formation during gastrulation in the mouse. Homozygous embryos have a defective allantois, degenerate or absent notochord and disrupted primitive streak and node. The neural tube is kinked and somite formation interrupted. The T gene has been cloned and is expressed during the early stages of gastrulation, being restricted to the primitive streak region, nascent mesoderm and notochord. Neither the sequence of the gene nor its expression pattern define its developmental function. To study the cell autonomy of the T mutation we have isolated and genetically characterized embryonic stem cell lines and studied their behaviour in chimaeras. T/+ embryonic stem cells form normal chimaeras, whereas T/T in equilibrium with +/+ chimaeras mimic the T/T mutant phenotype. The results indicate that the T gene acts cell autonomously in the primitive streak and notochord but may activate a signalling pathway involved in the specification of other mesodermal tissues.

DOI10.1038/353348a0
Alternate JournalNature
PubMed ID1922339