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CD41 is developmentally regulated and differentially expressed on mouse hematopoietic stem cells.

TitleCD41 is developmentally regulated and differentially expressed on mouse hematopoietic stem cells.
Publication TypeJournal Article
Year of Publication2011
AuthorsRobin C, Ottersbach K, Boisset J-C, Oziemlak A, Dzierzak E
JournalBlood
Volume117
Issue19
Pagination5088-91
Date Published2011 May 12
ISSN1528-0020
KeywordsAnimals, Aorta, Cell Separation, Female, Flow Cytometry, Gene Expression Regulation, Developmental, Hematopoiesis, Hematopoietic Stem Cells, Immunohistochemistry, Liver, Mice, Mice, Inbred C57BL, Mice, Transgenic, Placenta, Platelet Membrane Glycoprotein IIb, Pregnancy, Yolk Sac
Abstract

CD41 expression is associated with the earliest stages of mouse hematopoiesis. It is notably expressed on some cells of the intra-aortic hematopoietic clusters, an area where the first adult-repopulating hematopoietic stem cells (HSCs) are generated. Although it is generally accepted that CD41 expression marks the onset of primitive/definitive hematopoiesis, there are few published data concerning its expression on HSCs. It is as yet uncertain whether HSCs express CD41 throughout development, and if so, to what level. We performed a complete in vivo transplantation analysis with yolk sac, aorta, placenta, and fetal liver cells, sorted based on CD41 expression level. Our data show that the earliest emerging HSCs in the aorta express CD41 in a time-dependent manner. In contrast, placenta and liver HSCs are CD41⁻. Thus, differential and temporal expression of CD41 by HSCs in the distinct hematopoietic territories suggests a developmental/dynamic regulation of this marker throughout development.

DOI10.1182/blood-2011-01-329516
Alternate JournalBlood
PubMed ID21415271
PubMed Central IDPMC3109535
Grant ListG0800784 / / Medical Research Council / United Kingdom
R37 DK054077 / DK / NIDDK NIH HHS / United States
R37 DK51077 / DK / NIDDK NIH HHS / United States
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