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Bone morphogenic protein signalling suppresses differentiation of pluripotent cells by maintaining expression of E-Cadherin.

TitleBone morphogenic protein signalling suppresses differentiation of pluripotent cells by maintaining expression of E-Cadherin.
Publication TypeJournal Article
Year of Publication2013
AuthorsMalaguti M, Nistor PA, Blin G, Pegg A, Zhou X, Lowell S
JournalElife
Volume2
Paginatione01197
Date Published2013
ISSN2050-084X
Abstract

Bone morphogenic protein (BMP) signalling contributes towards maintenance of pluripotency and favours mesodermal over neural fates upon differentiation, but the mechanisms by which BMP controls differentiation are not well understood. We report that BMP regulates differentiation by blocking downregulation of Cdh1, an event that accompanies the earliest stages of neural and mesodermal differentiation. We find that loss of Cdh1 is a limiting requirement for differentiation of pluripotent cells, and that experimental suppression of Cdh1 activity rescues the BMP-imposed block to differentiation. We further show that BMP acts prior to and independently of Cdh1 to prime pluripotent cells for mesoderm differentiation, thus helping to reinforce the block to neural differentiation. We conclude that differentiation depends not only on exposure to appropriate extrinsic cues but also on morphogenetic events that control receptivity to those differentiation cues, and we explain how a key pluripotency signal, BMP, feeds into this control mechanism. DOI: http://dx.doi.org/10.7554/eLife.01197.001.

DOI10.7554/eLife.01197
Alternate JournalElife
PubMed ID24347544
PubMed Central IDPMC3865744