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Bmp4 and Noggin expression during early thymus and parathyroid organogenesis.

TitleBmp4 and Noggin expression during early thymus and parathyroid organogenesis.
Publication TypeJournal Article
Year of Publication2006
AuthorsPatel SR, Gordon J, Mahbub F, C Blackburn C, Manley NR
JournalGene Expr Patterns
Volume6
Issue8
Pagination794-9
Date Published2006 Oct
ISSN1567-133X
KeywordsAnimals, beta-Galactosidase, Bone Morphogenetic Protein 4, Bone Morphogenetic Proteins, Carrier Proteins, Embryo, Mammalian, Embryonic Development, Gene Expression Regulation, Developmental, Mice, Mice, Transgenic, Organogenesis, Parathyroid Glands, Recombinant Proteins, Thymus Gland
Abstract

The thymus and parathyroids originate from the third pharyngeal pouches, which form as endodermal outpocketings in the pharyngeal region beginning on embryonic day 9 (E9.0) of mouse development. Using organ-specific markers, we have previously shown that thymus and parathyroid-specific organ domains are established within the primordium prior to formation of the organs proper: Gcm2 expression defines the prospective parathyroid cells in the dorsal pouch from E9.5, while Foxn1 is expressed in the thymus domain from E11.25. Bmp (bone morphogenetic protein) signaling has been implicated in thymic epithelial cell differentiation and thymus organogenesis. In the present study, we report expression patterns of Bmp4 and Noggin, a Bmp4 antagonist, in the third pharyngeal pouch using two lacZ transgenic mouse strains. Results from this gene expression study revealed localization of Bmp4 expression to the ventral region of the third pharyngeal pouch endoderm at E10.5 and E11.5, in those cells that will express Foxn1 and form the thymus. Conversely, the expression of Noggin was confined to the dorsal region of the pouch and primordium at these stages, and thus appeared to be co-expressed with Gcm2 in the parathyroid domain. This represents the first detailed study of Bmp4 and Noggin expression during the early stages of thymus and parathyroid organogenesis.

DOI10.1016/j.modgep.2006.01.011
Alternate JournalGene Expr. Patterns
PubMed ID16517216
Grant ListG0300058 / / Medical Research Council / United Kingdom
HD35920 / HD / NICHD NIH HHS / United States
/ / Wellcome Trust / United Kingdom