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Analysis of reptilian APOBEC1 suggests that RNA editing may not be its ancestral function.

TitleAnalysis of reptilian APOBEC1 suggests that RNA editing may not be its ancestral function.
Publication TypeJournal Article
Year of Publication2011
AuthorsSeveri F, Chicca A, Conticello SG
JournalMol Biol Evol
Date Published2011 Mar
KeywordsAmino Acid Sequence, Animals, Apolipoproteins B, Biological Evolution, Cytidine Deaminase, Deamination, DNA, DNA, Bacterial, Escherichia coli, Gene Expression Regulation, Genetic Variation, Humans, Molecular Sequence Data, Mutation, Phylogeny, Protein Isoforms, Reptiles, RNA Editing, RNA, Messenger, Sequence Homology, Amino Acid

The Activation Induced Deaminase (AID)/APOBEC family of deaminases targeting nucleic acids arose at the beginning of the vertebrate radiation and further expanded in mammals. Following an analysis of the available genomic data, we report the identification of the APOBEC5, a novel group of paralogues in tetrapods. Moreover, we find bona fide homologues of Apolipoprotein B Editing Complex 1 (APOBEC1) in the genomes of anole lizard and zebra finch, thus implying its appearance prior to the divergence of the amniotes. apolipoprotein B editing complex 1 (APOBEC1), in contrast with other AID/APOBECs acting on DNA, is an RNA-editing enzyme that targets the transcript of Apolipoprotein B (ApoB), thereby causing the translation of a truncated form of the protein. 3'RACE experiments reveal a lizard APOBEC1-like molecule lacking a C-terminal region important for mammalian ApoB RNA editing. This observation pairs with the finding that lizard ApoB is not deaminated at the region corresponding to the mammalian site of editing. Similar to mammalian APOBEC1, the lizard protein is able to deaminate DNA in bacteria and shows a conserved mutational context. Although not precluding the possibility that lizard APOBEC1 acts on unknown mRNA targets, these findings suggest that its ability to target DNA predates its role in RNA editing.

Alternate JournalMol. Biol. Evol.
PubMed ID21172829
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