Steve Pollard

Position:

Group Leader and Professor of Stem Cell and Cancer Biology

Telephone:

0131 651 9500

Display email:

steven.pollard@ed.ac.uk

Biography:

2013 – present Group Leader, MRC Centre for Regenerative Medicine and Edinburgh Cancer Research Centre, University of Edinburgh
2009–2013 Group Leader, Samantha Dickson Brain Cancer Unit and UCL Cancer Institute
2006–2009 Beit Memorial Research Fellow and Kaye Fellow (Christ’s College), with Austin Smith, Wellcome Trust Centre for Stem Cell Research, University of Cambridge Neural stem cells and brain cancer
2002-2006 Postdoc with Prof Austin Smith FRS, Institute for Stem Cell Research, University of Edinburgh Conversion of pluripotent ES cells to multipotent NS cells.
1998–2002 PhD student with Dr Derek Stemple, Division of Developmental Biology, MRC National Institute for Medical Research, Mill Hill, London Molecular genetics of zebrafish early development.
1997 (summer) Research internship, Dept Developmental Neurobiology, St Jude Children’s Research Hospital, Memphis, USA
1994-1998 BSc Biochemistry, University of Bath

Additional publications:

Danovi D, Folarin A, Ender C, Elbatsh A, Engstrom PG, Gogolok S, Stricker SH, Yu D, KP U, Harvey KJ, Ferretti P, Paddison P, Bertone P, Smith A, Pollard SM. A high-content small molecule screen identifies sensitivity of glioblastoma stem cells to inhibition of polo-like kinase. PLoS One, 2013 Oct 30;8(10):e77053. doi:10.1371/journal.pone.0077053.
Stricker SH, Feber A, Kurian KM, Engstrom P, Tatashima Y, Watts C, Dirks P, Bertone P, Beck S, Smith A, Pollard SM. Widespread resetting of DNA methylation in glioblastoma initiating cells suppresses malignant cellular behaviour in a lineage dependent manner. Genes Dev. 2013. Mar 15;27(6):654-69. doi: 10.1101/
Danovi D, Folarin A, Baranowski B, and Pollard SM. High content screening of defined chemical libraries using normal and glioma-derived neural stem cells lines. Methods in Enzymol. 2012. 506, pp.311–329.
Pollard SM,$*, Yoshikawa K$, Clarke I, Danovi D, Stricker S, Russell R, Bayani J, Head R, Lee M, Bernstein M, Squire J, Smith A and Dirks P*. Glioma stem cell lines expanded in adherent culture have tumour-specific phenotypes and are suitable for chemical and genetic screens. Cell Stem Cell 2009 Jun 5;4(6):568-80. (* Joint Corresponding Author; $ Contributed Equally)

Publications

2019

Reprogramming of Fibroblasts to Oligodendrocyte Progenitor-like Cells Using CRISPR/Cas9-Based Synthetic Transcription Factors. Matjusaitis, Mantas, Wagstaff Laura J., Martella Andrea, Baranowski Bart, Blin Carla, Gogolok Sabine, Williams Anna C., and Pollard Steven M. , Stem Cell Reports, 2019 Nov 01, (2019)

2018

An efficient and scalable pipeline for epitope tagging in mammalian stem cells using Cas9 ribonucleoprotein. Dewari, Pooran Singh, Southgate Benjamin, Mccarten Katrina, Monogarov German, O'Duibhir Eoghan, Quinn Niall, Tyrer Ashley, Leitner Marie-Christin, Plumb Colin, Kalantzaki Maria, et al. , Elife, 2018 Apr 11, Volume 7, (2018)

2017

Polymeric glabrescione B nanocapsules for passive targeting of Hedgehog-dependent tumor therapy in vitro. Ingallina, Cinzia, Costa Pedro M., Ghirga Francesca, Klippstein Rebecca, Wang Julie T., Berardozzi Simone, Hodgins Naomi, Infante Paola, Pollard Steven M., Botta Bruno, et al. , Nanomedicine (Lond), 2017 Apr, Volume 12, Issue 7, p.711-728, (2017)

EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors. Martella, Andrea, Matjusaitis Mantas, Auxillos Jamie, Pollard Steven M., and Cai Yizhi , ACS Synth Biol, 2017 Apr 24, (2017)

Oncogenic activity of SOX1 in glioblastoma. Garcia, Idoia, Aldaregia Juncal, Vicentic Jelena Marjanovic, Aldaz Paula, Moreno-Cugnon Leire, Torres-Bayona Sergio, Carrasco-Garcia Estefania, Garros-Regulez Laura, Egaña Larraitz, Rubio Angel, et al. , Sci Rep, 2017 Apr 20, Volume 7, p.46575, (2017)

Elevated FOXG1 and SOX2 in glioblastoma enforces neural stem cell identity through transcriptional control of cell cycle and epigenetic regulators. Bulstrode, Harry, Johnstone Ewan, Marqués-Torrejón María Ángeles, Ferguson Kirsty M., Bressan Raul Bardini, Blin Carla, Grant Vivien, Gogolok Sabine, Gangoso Ester, Gagrica Sladjana, et al. , Genes Dev, 2017 Apr 15, Volume 31, Issue 8, p.757-773, (2017)

Efficient CRISPR/Cas9-assisted gene targeting enables rapid and precise genetic manipulation of mammalian neural stem cells. Bressan, Raul Bardini, Dewari Pooran Singh, Kalantzaki Maria, Gangoso Ester, Matjusaitis Mantas, Garcia-Diaz Claudia, Blin Carla, Grant Vivien, Bulstrode Harry, Gogolok Sabine, et al. , Development, 2017 Feb 15, Volume 144, Issue 4, p.635-648, (2017)

2016

Inactivation of the ATMIN/ATM pathway protects against glioblastoma formation. Blake, Sophia M., Stricker Stefan H., Halavach Hanna, Poetsch Anna R., Cresswell George, Kelly Gavin, Kanu Nnennaya, Marino Silvia, Luscombe Nicholas M., Pollard Steven M., et al. , Elife, 2016 Mar 17, Volume 5, (2016)

EphrinB2 drives perivascular invasion and proliferation of glioblastoma stem-like cells. Krusche, Benjamin, Ottone Cristina, Clements Melanie P., Johnstone Ewan R., Goetsch Katrin, Lieven Huang, Mota Silvia G., Singh Poonam, Khadayate Sanjay, Ashraf Azhaar, et al. , Elife, 2016 Jun 28, Volume 5, (2016)

STAR: a simple TAL effector assembly reaction using isothermal assembly. Gogolok, Sabine, Garcia-Diaz Claudia, and Pollard Steven M. , Sci Rep, 2016 Sep 12, Volume 6, p.33209, (2016)

Accelerating glioblastoma drug discovery: Convergence of patient-derived models, genome editing and phenotypic screening. O'Duibhir, Eoghan, Carragher Neil O., and Pollard Steven M. , Mol Cell Neurosci, 2016 Nov 04, (2016)

Quantitative stem cell biology: the threat and the glory. Pollard, Steven M. , Development, 2016 Nov 15, Volume 143, Issue 22, p.4097-4100, (2016)

Proteome and Secretome Characterization of Glioblastoma-Derived Neural Stem Cells. Okawa, Satoshi, Gagrica Sladjana, Blin Carla, Ender Christine, Pollard Steven M., and Krijgsveld Jeroen , Stem Cells, 2016 Nov 21, (2016)

Differentiation therapy for glioblastoma – too many obstacles? Carén, Helena, Beck Stephan, and Pollard Steven M. , Molecular & Cellular Oncology, Mar-03-2016, Volume 3, Issue 2, p.e1124174, (2016)

Mammalian Synthetic Biology: Time for Big MACs. Martella, Andrea, Pollard Steven M., Dai Junbiao, and Cai Yizhi , ACS Synth Biol, 2016 Apr 20, (2016)

mTOR inhibition decreases SOX2-SOX9 mediated glioma stem cell activity and temozolomide resistance. Garros-Regulez, Laura, Aldaz Paula, Arrizabalaga Olatz, Moncho-Amor Veronica, Carrasco-Garcia Estefania, Manterola Lorea, Moreno-Cugnon Leire, Barrena Cristina, Villanua Jorge, Ruiz Irune, et al. , Expert Opin Ther Targets, 2016 Apr, Volume 20, Issue 4, p.393-405, (2016)

Give it a REST! Pollard, Steven M., and M Marqués-Torrejón Ángeles , Elife, 2016, Volume 5, (2016)

2015

Glioblastoma Stem Cells Respond to Differentiation Cues but Fail to Undergo Commitment and Terminal Cell-Cycle Arrest. Carén, Helena, Stricker Stefan H., Bulstrode Harry, Gagrica Sladjana, Johnstone Ewan, Bartlett Thomas E., Feber Andrew, Wilson Gareth, Teschendorff Andrew E., Bertone Paul, et al. , Stem Cell Reports, 2015 Oct 21, (2015)

Genome-wide CRISPR-Cas9 Screens Reveal Loss of Redundancy between PKMYT1 and WEE1 in Glioblastoma Stem-like Cells. Toledo, Chad M., Ding Yu, Hoellerbauer Pia, Davis Ryan J., Basom Ryan, Girard Emily J., Lee Eunjee, Corrin Philip, Hart Traver, Bolouri Hamid, et al. , Cell Rep, 2015 Dec 7, (2015)

2014

Reprogramming cancer cells to pluripotency: an experimental tool for exploring cancer epigenetics. Stricker, Stefan, and Pollard Steven M. , Epigenetics, 2014 Jun, Volume 9, Issue 6, p.798-802, (2014)

2013

Cohesin-mediated interactions organize chromosomal domain architecture. Sofueva, Sevil, Yaffe Eitan, Chan Wen-Ching, Georgopoulou Dimitra, Rudan Matteo Vietri, Mira-Bontenbal Hegias, Pollard Steven M., Schroth Gary P., Tanay Amos, and Hadjur Suzana , EMBO J, 2013 Dec 11, Volume 32, Issue 24, p.3119-29, (2013)

Genome-wide RNAi screens in human brain tumor isolates reveal a novel viability requirement for PHF5A. Hubert, Christopher G., Bradley Robert K., Ding Yu, Toledo Chad M., Herman Jacob, Skutt-Kakaria Kyobi, Girard Emily J., Davison Jerry, Berndt Jason, Corrin Philip, et al. , Genes Dev, 2013 May 1, Volume 27, Issue 9, p.1032-45, (2013)

Widespread resetting of DNA methylation in glioblastoma-initiating cells suppresses malignant cellular behavior in a lineage-dependent manner. Stricker, Stefan H., Feber Andrew, Engström Pär G., Carén Helena, Kurian Kathreena M., Takashima Yasuhiro, Watts Colin, Way Michael, Dirks Peter, Bertone Paul, et al. , Genes Dev, 2013 Mar 15, Volume 27, Issue 6, p.654-69, (2013)

In vitro expansion of fetal neural progenitors as adherent cell lines. Pollard, Steven M. , Methods Mol Biol, 2013, Volume 1059, p.13-24, (2013)

Cancer-Specific requirement for BUB1B/BUBR1 in human brain tumor isolates and genetically transformed cells. Ding, Yu, Hubert Christopher G., Herman Jacob, Corrin Philip, Toledo Chad M., Skutt-Kakaria Kyobi, Vazquez Julio, Basom Ryan, Zhang Bin, Risler Jennifer K., et al. , Cancer Discov, 2013 Feb, Volume 3, Issue 2, p.198-211, (2013)

The good, the bad and the ugly: epigenetic mechanisms in glioblastoma. Carén, Helena, Pollard Steven M., and Beck Stephan , Mol Aspects Med, 2013 Jul-Aug, Volume 34, Issue 4, p.849-62, (2013)

A high-content small molecule screen identifies sensitivity of glioblastoma stem cells to inhibition of polo-like kinase 1. Danovi, Davide, Folarin Amos, Gogolok Sabine, Ender Christine, Elbatsh Ahmed M. O., Engström Pär G., Stricker Stefan H., Gagrica Sladjana, Georgian Ana, Yu Ding, et al. , PLoS One, 2013, Volume 8, Issue 10, p.e77053, (2013)

2012

Digital transcriptome profiling of normal and glioblastoma-derived neural stem cells identifies genes associated with patient survival. Engström, Pär G., Tommei Diva, Stricker Stefan H., Ender Christine, Pollard Steven M., and Bertone Paul , Genome Med, 2012, Volume 4, Issue 10, p.76, (2012)

High content screening of defined chemical libraries using normal and glioma-derived neural stem cell lines. Danovi, Davide, Folarin Amos A., Baranowski Bart, and Pollard Steven M. , Methods Enzymol, 2012, Volume 506, p.311-29, (2012)

Capture of neuroepithelial-like stem cells from pluripotent stem cells provides a versatile system for in vitro production of human neurons. Falk, Anna, Koch Philipp, Kesavan Jaideep, Takashima Yasuhiro, Ladewig Julia, Alexander Michael, Wiskow Ole, Tailor Jignesh, Trotter Matthew, Pollard Steven M., et al. , PLoS One, 2012, Volume 7, Issue 1, p.e29597, (2012)

2011

Non-immortalized human neural stem (NS) cells as a scalable platform for cellular assays. Hook, Lilian, Vives Joaquim, Fulton Norma, Leveridge Mathew, Lingard Sarah, Bootman Martin D., Falk Anna, Pollard Steven M., Allsopp Timothy E., Dalma-Weiszhausz Dennise, et al. , Neurochem Int, 2011 Sep, Volume 59, Issue 3, p.432-44, (2011)

Interplay between FGF2 and BMP controls the self-renewal, dormancy and differentiation of rat neural stem cells. Sun, Yirui, Hu Jin, Zhou Liangfu, Pollard Steven M., and Smith Austin , J Cell Sci, 2011 Jun 1, Volume 124, Issue Pt 11, p.1867-77, (2011)

Sox2 and Pax6 maintain the proliferative and developmental potential of gliogenic neural stem cells In vitro. Gómez-López, Sandra, Wiskow Ole, Favaro Rebecca, Nicolis Silvia K., Pollard Steven M., and Smith Austin , Glia, 2011 Nov, Volume 59, Issue 11, p.1588-99, (2011)

2010

Imaging-based chemical screens using normal and glioma-derived neural stem cells. Danovi, Davide, Falk Anna, Humphreys Peter, Vickers Richard, Tinsley Jon, Smith Austin G., and Pollard Steven M. , Biochem Soc Trans, 2010 Aug, Volume 38, Issue 4, p.1067-71, (2010)

2009

CD133 (Prominin) negative human neural stem cells are clonogenic and tripotent. Sun, Yirui, Kong Weiqing, Falk Anna, Hu Jin, Zhou Liangfu, Pollard Steven M., and Smith Austin , PLoS One, 2009, Volume 4, Issue 5, p.e5498, (2009)

Preview. A shore sign of reprogramming. Pollard, Steven M., Stricker Stefan H., and Beck Stephan , Cell Stem Cell, 2009 Dec 4, Volume 5, Issue 6, p.571-2, (2009)

Glioma stem cell lines expanded in adherent culture have tumor-specific phenotypes and are suitable for chemical and genetic screens. Pollard, Steven M., Yoshikawa Koichi, Clarke Ian D., Danovi Davide, Stricker Stefan, Russell Roslin, Bayani Jane, Head Renee, Lee Marco, Bernstein Mark, et al. , Cell Stem Cell, 2009 Jun 5, Volume 4, Issue 6, p.568-80, (2009)

The methyl-CpG binding proteins Mecp2, Mbd2 and Kaiso are dispensable for mouse embryogenesis, but play a redundant function in neural differentiation. Caballero, Isabel M., Hansen Janne, Leaford Donna, Pollard Steven M., and Hendrich Brian , PLoS One, 2009, Volume 4, Issue 1, p.e4315, (2009)

2008

REST regulates distinct transcriptional networks in embryonic and neural stem cells. Johnson, Rory, Teh Christina Hui-leng, Kunarso Galih, Wong Kee Yew, Srinivasan Gopalan, Cooper Megan L., Volta Manuela, Chan Sarah Su-ling, Lipovich Leonard, Pollard Steven M., et al. , PLoS Biol, 2008 Oct 28, Volume 6, Issue 10, p.e256, (2008)

A diacylglycerol lipase-CB2 cannabinoid pathway regulates adult subventricular zone neurogenesis in an age-dependent manner. Goncalves, Maria Beatriz, Suetterlin Philipp, Yip Ping, Molina-Holgado Francisco, Walker Deborah J., Oudin Madeleine J., Zentar Marc P., Pollard Steven M., Yáñez-Muñoz Rafael J., Williams Gareth, et al. , Mol Cell Neurosci, 2008 Aug, Volume 38, Issue 4, p.526-36, (2008)

Long-term tripotent differentiation capacity of human neural stem (NS) cells in adherent culture. Sun, Yirui, Pollard Steven M., Conti Luciano, Toselli Mauro, Biella Gerardo, Parkin Georgina, Willatt Lionel, Falk Anna, Cattaneo Elena, and Smith Austin , Mol Cell Neurosci, 2008 Jun, Volume 38, Issue 2, p.245-58, (2008)

Fibroblast growth factor induces a neural stem cell phenotype in foetal forebrain progenitors and during embryonic stem cell differentiation. Pollard, Steven M., Wallbank Richard, Tomlinson Simon R., Grotewold Lars, and Smith Austin , Mol Cell Neurosci, 2008 Jul, Volume 38, Issue 3, p.393-403, (2008)

2007

Tripotential differentiation of adherently expandable neural stem (NS) cells. Glaser, Tamara, Pollard Steven M., Smith Austin, and Brüstle Oliver , PLoS One, 2007, Volume 2, Issue 3, p.e298, (2007)

Investigating radial glia in vitro. Pollard, Steven M., and Conti Luciano , Prog Neurobiol, 2007 Sep, Volume 83, Issue 1, p.53-67, (2007)

2006

Essential and overlapping roles for laminin alpha chains in notochord and blood vessel formation. Pollard, Steven M., Parsons Michael J., Kamei Makoto, Kettleborough Ross N. W., Thomas Kevin A., Pham Van N., Bae Moon-Kyoung, Scott Annabelle, Weinstein Brant M., and Stemple Derek L. , Dev Biol, 2006 Jan 1, Volume 289, Issue 1, p.64-76, (2006)

Exploitation of adherent neural stem cells in basic and applied neurobiology. Pollard, Steven M., Conti Luciano, and Smith Austin , Regen Med, 2006 Jan, Volume 1, Issue 1, p.111-8, (2006)

Nanog promotes transfer of pluripotency after cell fusion. Silva, Jose, Chambers Ian, Pollard Steven M., and Smith Austin , Nature, 2006 Jun 22, Volume 441, Issue 7096, p.997-1001, (2006)

Adherent neural stem (NS) cells from fetal and adult forebrain. Pollard, Steven M., Conti Luciano, Sun Yirui, Goffredo Donato, and Smith Austin , Cereb Cortex, 2006 Jul, Volume 16 Suppl 1, p.i112-20, (2006)

Reprogramming efficiency following somatic cell nuclear transfer is influenced by the differentiation and methylation state of the donor nucleus. Blelloch, Robert, Wang Zhongde, Meissner Alex, Pollard Steven M., Smith Austin, and Jaenisch Rudolf , Stem Cells, 2006 Sep, Volume 24, Issue 9, p.2007-13, (2006)

Neural stem cells, neurons, and glia. Pollard, Steven M., Benchoua Alexandra, and Lowell Sally , Methods Enzymol, 2006, Volume 418, p.151-69, (2006)

2005

Niche-independent symmetrical self-renewal of a mammalian tissue stem cell. Conti, Luciano, Pollard Steven M., Gorba Thorsten, Reitano Erika, Toselli Mauro, Biella Gerardo, Sun Yirui, Sanzone Sveva, Ying Qi-Long, Cattaneo Elena, et al. , PLoS Biol, 2005 Sep, Volume 3, Issue 9, p.e283, (2005)

2002

Zebrafish mutants identify an essential role for laminins in notochord formation. Parsons, Michael J., Pollard Steven M., Saúde Leonor, Feldman Benjamin, Coutinho Pedro, Hirst Elizabeth M. A., and Stemple Derek L. , Development, 2002 Jul, Volume 129, Issue 13, p.3137-46, (2002)

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